product name：Rabbit Aortic Fibroblasts（兔主動脈成纖維細(xì)胞）；

cat no：ABC-TC4055；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description：Rabbit Primary Aortic Fibroblasts（兔主動脈成纖維細(xì)胞）from AcceGen are isolated from aortic tissue of New Zealand White Rabbits. Rabbit Primary Aortic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Rabbit Primary Aortic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-6 passages at a split ratio of 1:3 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.